DNA Methyltransferase 3L (160-387 aa), Human recombinant

DNMT3L

The DNA methyltransferase 3 (DNMT3) family of DNMT proteins perform intial methylation of cytosines to form 5-methylcytosine at CpG (cytosine-phosphate-guanine) sites on the DNA. Once methylation is established on DNA, DNMT1 protein is responsible for maintaining the CpG methylation pattern during chromosome replication. The DNMT3 family includes the two active members, DNMT3a and DNMT3b, and the regulatory factor DNMT3-like protein (DNMT3L). DNMT3L contains a cysteine-rich, DNMT3/ATRX homology or ATRX-DNMT3-DNMT3L (ADD) domain; however it lacks the proline-tryptophan-tryptophan-proline (PWWP) domain, required for non-specific DNA binding. The ADD domain of DNMT3L is responsible for specifically binding to unmethylated histone H3 lysine 4 (H3K4me0). DNMT3L also lacks the conserved residues in the C-terminal DNA MTase domain of DNMT3a and 3b that are required for activity. However, DNMT3L is required to stimulate the DNA methylation activity of DNMT3a and 3b through interactions with the catalytic domain of DNMT3a and 3b.

N/A

53.2 kDa (160-387 aa + NT GST Tag)

DNA Methyltransferase 3L (160-387 aa), Human recombinant

DNMT3-Like Protein; DNMT3L

29947

DNMT3L

Human

Q9UJW3

Yes

E. coli

≥95%

SDS-PAGE, HPLC

N/A

N/A

N/A

N/A

N/A

N/A

-80°C

dry ice

Liquid

50 mM Tris, pH 8.0, containing 150 mM sodium chloride and 20% glycerol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants