Urokinase, human recombinant

Catalog number
7696-10
Name
Urokinase, human recombinant
Size
10 µg
Price
305.00 EUR
Supplier
Biovision
Order

Details

Synonyms
Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
Alternates names
Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
Taglines
A potent marker of invasion and metastasis in a variety of human cancers
Biomolecule/Target
Urokinase
NCBI Gene ID #.
5328
NCBI Gene Symbol
PLAU
Gene Source
Human
Accession #
P00749
Recombinant
Yes
Source
E. coli.
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) (Cat # K728-100).
Biological activity
>1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
Results
>1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.)
Molecular Weight
The active Urokinase is a two-chain glycoprotein and has a calculated MW of 49.3 kDa. DTT-reduced Protein migrates as two bands corresponding to the two chains with the molecular mass of 28.3 and 21 kDa.
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from proprietary buffer.
Reconstitution Instructions
Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at –80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants