Active SIRT2, human recombinant

Catalog number
7698-50
Name
Active SIRT2, human recombinant
Size
50 µg
Price
525.00 EUR
Supplier

Details

Synonyms
NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.
Alternates names
NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.
Taglines
Human SIRT2 from E.Coli, AOF, ≥90% pure, biologically active.
Biomolecule/Target
SIRT2
NCBI Gene ID #.
22933
NCBI Gene Symbol
SIRT2
Gene Source
Human
Accession #
Q8IXJ6
Recombinant
Yes
Source
E. coli.
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
Activity of SIRT2 was tested with BioVision’s Sirtuin Activity assay kit, Cat # K324-100, using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53.
Biological activity
≥ 4 mU/mg
Results
≥ 4 mU/mg
Molecular Weight
Human SIRT2 is a 60 kDa (2 to 389 aa + NT His Tag) protein
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder.
Physical form description
Human Sirtuin 2 is lyophilized from 50 mM Tris, pH 8.0.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile dH2O to a concentration of 0.1 – 1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants