Sulfo-NHS-Biotin for cell activation
Catalog number
2322-1set
Name
Sulfo-NHS-Biotin for cell activation
Size
1 set (10 x 1 mg)
Price
175.00 EUR
Supplier
Details
Description
KEY FEATURES: • Sulfo-NHS-Biotin is amine-reactive and has a spacer arm length of 13.5 Å. • Water-soluble; can be used under physiologic conditions • The amide bond formed is permanent and the alkyl spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
Peptide sequence
N/A
CAS number
119616-38-5
Molecular weight
443.43
Category
Biochemicals
Other name
Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfosuccinimidobiotin; Sulfo NHS Biotin;Sulfosuccinimidyl biotin; N-hydroxysulfosuccinimidyl biotin; N-hydroxysulfosuccinimido biotin
Molecular formula
C₁₄H₁₈O₈N₃S₂Na
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥85% by NMR
Reconstitute instructions
H₂O (~ 20 mg/ml)
Storage condition
-20 ̊C
Shipping condition
Gel Pack
Maximum time for storage
36 months
Storage instructions
Protect from moisture
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.
