Sulfo-NHS-SS-Biotin for cell activation
Catalog number
2323-50
Name
Sulfo-NHS-SS-Biotin for cell activation
Size
50 mg
Price
186.00 EUR
Supplier
Details
Description
KEY FEATURES: • Sulfo-NHS-SS-Biotin is amine-reactive and • Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester • Water-soluble; can be used under physiologic conditions • It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP APPLICATIONS: • Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
Peptide sequence
N/A
CAS number
325143-98-4
Molecular weight
606.69
Category
Biochemicals
Other name
Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate; Biotin disulfide N-Hydroxysulfosuccinimide ester
Molecular formula
C₁₉H₂₇O₉N₄S₄Na
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥80% by NMR
Reconstitute instructions
H₂O (~ 6 mg/ml)
Storage condition
+4 ̊C
Shipping condition
Gel Pack
Maximum time for storage
36 months
Storage instructions
Protect from moisture
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.
