Sulfo-LC-SPDP for cell activation

KEY FEATURES: • Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed • Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm • More water-soluble than the corresponding LC-SPDP • Membrane-impermeable which enables crosslinking on cell surface • The cleavable spacer arm enables separation of cross-linked products APPLICATIONS: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

N/A

N/A

527.57

Biochemicals

Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate

C₁₈H₂₂O₈N₃S₃Na

White to Off-white Solid

N/A

No

No

≥90% by NMR

H₂O (~ 6 mg/ml)

-20 ̊C

Gel Pack

36 months

Protect from moisture

cell

For cells, cell lines and tissues in culture till half confluency.