Glucokinase, human liver, recombinant
Catalog number
7776-10
Name
Glucokinase, human liver, recombinant
Size
10 μg
Price
295.00 EUR
Supplier
Details
Synonyms
NA
Alternates names
Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Taglines
Plays a key role in the communication of cells with their microenvironment
Biomolecule/Target
NA
NCBI Gene ID #.
2645
NCBI Gene Symbol
GCK
Gene Source
Human liver
Accession #
P35557
Recombinant
Yes
Source
E. coli.
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
662 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.
Biological activity
N/A
Results
N/A
Molecular Weight
53.4 kDa
Storage Temp.
-80°C
Shipping
dry ice
Shelf Life
1 year
Concentration
1 mg/ml
Appearance
Liquid
Physical form description
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Reconstitution Instructions
N/A
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Tissue
liver
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
