Sulfo-NHS-LC-Biotin for cell activation

KEY FEATURES: • Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling • More water-soluble than the corresponding NHS compound without the sulfo group • Membrane-impermeable • Forms permanent amide bonds and the spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent

N/A

127062-22-0

556.59

Biochemicals

Sulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt

C₁₄H₁₈O₈N₃S₂Na

White to Off-white Solid

N/A

No

No

≥90% by NMR

H₂O (~ 20 mg/ml)

-20 ̊C

Gel Pack

36 months

Protect from moisture

cell

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

For cells, cell lines and tissues in culture till half confluency.