Sulfo-NHS-LC-Biotin for cell activation

Catalog number
2326-100
Name
Sulfo-NHS-LC-Biotin for cell activation
Size
100 mg
Price
271.00 EUR
Supplier

Details

Description
KEY FEATURES: • Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling • More water-soluble than the corresponding NHS compound without the sulfo group • Membrane-impermeable • Forms permanent amide bonds and the spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
Peptide sequence
N/A
CAS number
127062-22-0
Molecular weight
556.59
Category
Biochemicals
Other name
Sulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
Molecular formula
C₁₄H₁₈O₈N₃S₂Na
Physical appearance
White to Off-white Solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥90% by NMR
Reconstitute instructions
H₂O (~ 20 mg/ml)
Storage condition
-20 ̊C
Shipping condition
Gel Pack
Maximum time for storage
36 months
Storage instructions
Protect from moisture
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.