Biotin-LC-LC-NHS for cell activation
Catalog number
2346-250
Name
Biotin-LC-LC-NHS for cell activation
Size
250 mg
Price
588.00 EUR
Supplier
Details
Description
Biotin-LC-LC-NHS is a useful reagent for biotinylation of primary amines under mild conditions (pH~6.5-8.5). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 14-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance in binding avidin or streptavidin to some biotinylated compounds and increase sensitivity for detection.
Peptide sequence
N/A
CAS number
89889-52-1
Molecular weight
567.7
Category
Biochemicals
Other name
6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid, succinimidyl ester; Biotin-XX-NHS
Molecular formula
C₂₆H₄₁NO₇S
Physical appearance
White solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥95% by HPLC
Reconstitute instructions
DMF (~ 25 mg/ml)
Storage condition
-20 ̊C
Shipping condition
gel pack
Maximum time for storage
36 months
Storage instructions
Protect from air and moisture
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.
