Human CellExp™CNTFR/CNTFR-alpha, human recombinant

Catalog number

7836-50

Size

50 μg

Price

659.00 EUR

Supplier

Biovision

Order

Details

Target

CNTFR/CNTFR-alpha

Background

Ciliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.

Concentration

N/A

Molecular weight

This protein rh CNTFR alpha is fused with polyhistidine tag at the C-terminus, has a calculated MW of 36.9 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 45-48 kDa due to glycosylation.

Synonym

CNTFR, CNTF R alpha

Other name

CNTFR, CNTF R alpha

NCBI gene number

1271

NCBI gene

CNTFR

Gene source

Human

NCBI number

P26992

Recombinant

Yes

Source

HEK 293 cells

Purification

≥95%

Tested applications

SDS-PAGE, N/A

Level of endotoxin

< 1.0 EU per μg of the rh CNTFR alpha by the LAL method.

Tested activity

Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Reesults

Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

gel pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Description

The CellExp™CNTFR/CNTFR-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants