EZCut™ TEV Protease, Recombinant

TEV Protease

BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

1 mg/ml

28.6 kDa (2038–2279 aa + C-terminal poly-his tag).

Nuclear inclusion protein A, NIa protein

Nuclear inclusion protein A, NIa protein

N/A

N/A

N/A

N/A

Yes

E. coli

≥95%

SDS-PAGE, HPLC

N/A

The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.

The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.

≥10,000 units/mg

N/A

One unit is defined as the amount of EZCut™ TEV Protease required to cleave >90% of 10 µg a control protein substrate (containing an ENLYFQS sequence at N-terminus) in 1 h at 37°C.

-80°C

dry ice

Liquid

1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0

N/A

2038–2279 aa

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Recombinants or rec. proteins

recombinants