EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant

SUMO Protease 1

SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).

0.1 mg/ml (100 U/µl)

28.7 kDa (403-621 aa + N-terminal Poly-His tag).

Ulp1 peptidase, SUMO Protease

Ulp1 peptidase, SUMO Protease

856087

ULP1

Yeast

Q02724

Yes

E. coli

≥90%

SDS-PAGE , N/A

N/A

The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.

The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.

≥ 1 X 106 units/mg.

N/A

One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.

-80°C

Dry ice

Liquid

0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0

N/A

403-621 aa + N-terminal Poly-His tag

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

histidine

Recombinants or rec. proteins

recombinants