EZLys™ Lysozyme, Human

Catalog number
8005-5G
Name
EZLys™ Lysozyme, Human
Size
5 g
Price
262.00 EUR
Supplier

Details

Background
EZLys™ Lysozyme is a recombinant human lysozyme expressed and purified in an animal-free system (rice). It has an identical amino acid sequence and physic-chemical properties of native lysozyme. The enzyme cleaves b (1,4) linkage between N-acetylglucosamine and N-acetylmuramic acid of the peptidoglycan component in the bacterial cell wall. It exhibits four times more activity than commercial chicken lysozyme and is useful in the recovery of proteins expressed in bacteria (e.g. E. coli). EZ Lysozyme is designed for gentle cell lysis which leads to a minimal risk of protein denaturation.
Concentration
N/A
Molecular weight
N/A
Synonym
Protein Extraction
Other name
Lysozyme, EZLys™ , 1,4-beta-N-acetylmuramidase C
NCBI gene number
N/A
NCBI gene
N/A
Gene source
N/A
NCBI number
N/A
Recombinant
Yes
Source
E. coli
Purification
≥85%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
>100,000 units/mg protein. A unit (U) is defined as the amount of enzyme which will reduce the absorbance at 450 nm, using a suspension of Micrococcus lysodeikticus as a substrate in a 2.6 ml reaction volume, by 0.001 absorbance units per min at pH 6.24 and 25°C.
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Off- white powder
Physical properties
Off- white powder
How to reconstitute
Clear solution at 10 mg/ml in PBS, pH 7.4 or H₂O
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.