Human CellExp™ TIGIT, human recombinant
Catalog number
8015-10
Name
Human CellExp™ TIGIT, human recombinant
Size
10 µg
Price
349.00 EUR
Supplier
Details
Target
TIGIT
Background
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Concentration
N/A
Molecular weight
This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15 kDa. The predicted N-terminus is Met 22. DTT-reduced Protein migrates as 18-24 kDa in SDS-PAGE due to glycosylation.
Synonym
TIGIT, VSIG9, VSTM3
Other name
TIGIT, VSIG9, VSTM3
NCBI gene number
201633
NCBI gene
TIGIT
Gene source
Human
NCBI number
Q495A1
Recombinant
Yes
Source
HEK 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
< 1.0 EU per μg of the protein by the LAL method.
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Aa sequence
Met 22 – Pro 141
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
