Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinant

Catalog number
P1004-20
Name
Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinant
Size
20 μg
Price
257.00 EUR
Supplier
Biovision
Order

Details

Target
Fc gamma RI
Background
Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
Concentration
N/A
Molecular weight
The protein has a calculated MW of 34.3 kDa. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
Synonym
High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64, FCGR1A
Other name
High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64 ,FCGR1A
NCBI gene number
2209
NCBI gene
FCGR1A
Gene source
Human
NCBI number
P12314
Recombinant
Yes
Source
HEK 293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Tested activity
Fc gamma RI binding ability is measured by functional ELISA.
Biological activity
Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
Reesults
N/A
Binding ability
Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Dry powder
Physical properties
Lyophilized powder
How to reconstitute
Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
Aa sequence
Gln 16 – Pro 288
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants