Exonuclease-Free Klenow
Catalog number
9219-500
Name
Exonuclease-Free Klenow
Size
500 UN
Price
202.00 EUR
Supplier
Details
Background
The large fragment of DNA polymerase I from E. coli, Klenow, has been genetically engineered to eliminate the 3'→5' exonuclease activity normally associated with this polymerase. The enzyme also lacks the 5' to 3' exonuclease activity of intact DNA polymerase I.
Concentration
N/A
Molecular weight
68.0 kDa
Synonym
Deoxynucleoside triphosphate; DNA Deoxynucleosidyltransferase, Large Fragment of DNA Polymerase I; Exonuclease-Free Klenow Enzyme.
Other name
Deoxynucleoside triphosphate; DNA Deoxynucleosidyltransferase, Large Fragment of DNA Polymerase I; Exonuclease-Free Klenow Enzyme.
Recombinant
Yes
Source
E. coli strain containing an overproducing clone of E. coli Exonuclease-free Klenow fragment.
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
10 unit/μl
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of total deoxyribonucleotides into an acid-precipitable material in 30 min at 37°C.
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Liquid
Physical properties
Recombinant Exonuclease-Free Klenow enzyme in 20 mM Tris-HCI (pH 7.5), 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 50% glycerol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
