Optikinase
Catalog number
9221-250
Name
Optikinase
Size
250 UN
Price
186.00 EUR
Supplier
Details
Background
OptiKinase™ is a modified version of T4 Polynucleotide Kinase (T4 PNK) which provides greater 32P or 33P labeling efficiency of DNA.
Concentration
N/A
Molecular weight
Homotetramer of 140 kDa (4 x 35 kDa)
Synonym
Phosphatase minus mutant of T4 PNK
Other name
Phosphatase minus mutant of T4 PNK
Recombinant
Yes
Source
E. coli strain containing a modified clone of T4 Polynucleotide Kinase that has full kinase activity but lacks phosphatase activity.
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
10 unit/μl
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit is the amount of enzyme required to incorporate 1 nmol of phosphate from radiolabeled ATP into DNA substrate in 30 min at 37°C.
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Liquid
Physical properties
Phosphatase minus mutant of T4 Polynucleotide Kinase in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA, 50% glycerol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
