DNA Polymerase I

Catalog number
9222-100
Name
DNA Polymerase I
Size
100 UN
Price
153.00 EUR
Supplier

Details

Background
DNA Polymerase I is a DNA dependent 5'→3' polymerase with inherent 3'→5'  and 5'→3'  exonuclease activity.
Concentration
N/A
Molecular weight
109 kDa
Synonym
Deoxynucleoside Triphosphate; DNA Deoxynucleosidyltransferase
Other name
Deoxynucleoside Triphosphate: DNA Deoxynucleosidyltransferase
Recombinant
Yes
Source
E. coli strain containing an overproducing clone of E. coli DNA Polymerase I.
Purification
≥90%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
10 unit/μl
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit is the amount of enzyme required to catalyze the conversion of 10 nmol of total deoxyribonucleotides into an acid-insoluble form in 30 min at 37°C using activated DNA as the template-primer.
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Liquid
Physical properties
DNA Polymerase I in 50mM potassium phosphate (pH 7.0), 1.0mM DTT, 50% glycerol, 1mM EDTA.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans