VeriScript Reverse Transcriptase

VeriScript Reverse Transcriptase is an engineered version of M-MLV Reverse Transcriptase with reduced RNase H activity and increased thermostability, allowing for first-strand cDNA synthesis with more full length cDNAs up to 12.5 kb. This DNA polymerase can synthesize complementary DNA from single-stranded DNA, RNA, or a RNA:DNA hybrid.

N/A

N/A

Verscript

Verscript

Yes

Recombinant

≥95%

SDS-PAGE, N/A

N/A

200 unit/μl

N/A

N/A

N/A

One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer.

-20°C

Gel pack

Liquid

Recombinant VeriScript Reverse Transcriptase in 20mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Igepal CA-630, and 50% Glycerol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.

reverse transcription