Human CellExp™ 4-1BB / TNFRSF9, Human recombinant

CD137

4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF

N/A

This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 18.1 kDa. The protein migrates as 28-35 kDa in SDS-PAGE due to glycosylation.

TNFRSF9,4-1BB,CD137,CDw137,ILA

TNFRSF9,4-1BB,CD137,CDw137,ILA

3604

TNFRSF9

Human

NP_001552.2

Yes

HEK 293 cells

≥92%

SDS-PAGE, N/A

< 1 EU/μg

Measured by its binding ability in a functional ELISA

Immobilized Human 4-1BB, His Tag at 100 ng/Ml (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.1-2.5 ng/mL.

N/A

Immobilized Human 4-1BB, His Tag at 100 ng/Ml (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.1-2.5 ng/mL.

N/A

-20°C

Gel pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants