Human CellExp™ BTLA, Human recombinant

Catalog number

9228-50

Size

50 µg

Price

735.00 EUR

Supplier

Biovision

Order

Details

Target

CD273

Background

B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

Concentration

N/A

Molecular weight

This protein is fused with a human IgG1 Fc tag at C-terminus and has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.

Synonym

BTLA, CD272

Other name

BTLA, CD272

NCBI gene number

151888

NCBI gene

BTLA

Gene source

Human

NCBI number

NP_001078826.1

Recombinant

Yes

Source

HEK 293 cells

Purification

≥92%

Tested applications

SDS-PAGE, N/A

Level of endotoxin

< 1 EU/μg

Tested activity

Measured by its binding ability in a functional ELISA

Biological activity

Immobilized Human HVEM, C-Twin Streptavidin Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.

Reesults

N/A

Binding ability

Immobilized Human HVEM, C-Twin Streptavidin Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.

Unit

N/A

Storage condition

-20°C

Shipping under

Gel pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants