Human CellExp™ Human CD40/ TNFRSF5, Human recombinant

Catalog number

9230-10

Size

10 µg

Price

1105.00 EUR

Supplier

Biovision

Order

Details

Target

CD40

Background

Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).

Concentration

N/A

Molecular weight

This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 20 kDa. The protein migrates as 32 kDa in SDS-PAGE due to glycosylation.

Synonym

CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50

Other name

CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50

NCBI gene number

958

NCBI gene

CD40

Gene source

Human

NCBI number

AAH12419.1

Recombinant

Yes

Source

HEK 293 cells

Purification

>95%

Tested applications

SDS-PAGE, N/A

Level of endotoxin

< 1 EU/μg

Tested activity

Measured by its binding ability in a functional ELISA.

Biological activity

Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.

Reesults

N/A

Binding ability

Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.

Unit

N/A

Storage condition

-20°C

Shipping under

Gel pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants