BenzAlt™ Binuclease (Liquid)

Catalog number
9215-50
Name
BenzAlt™ Binuclease (Liquid)
Size
50 KU
Price
458.00 EUR
Supplier

Details

Background
BenzAlt™ Binuclease is a genetically engineered non-specific endonuclease from Serratia marcescens gene. It is a perfect alternative for Benzonase (BenzAlt™ ) and is very cost effective. Functionally, this promiscuous nuclease digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. BenzAlt™ Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond at all positions among a nucleic acid chain. The enzyme is produced and purified from eukaryotic yeast cells, without contamination of endotoxin from prokaryotic cells and is highly stable even under harsh industrial conditions. It has no detectable proteinase or esterase activity. BenzAlt™ Binuclease is an ideal tool enzyme in protein purification and DNA/RNA contamination from purified proteins.
Concentration
N/A
Molecular weight
27.9 kDa
Synonym
BenzAltBinuclease, BenzAlt™ Binuclease, Binuclease, Benzonase alternative, endonuclease, Serratia marcescens binuclease, binuclease, benaltbinuclease, unrestricted endonuclease
Other name
Serratia marcescens binuclease; unrestricted endonuclea
Recombinant
Produced and purified from eukaryotic yeast cells
Source
Serratia marcescens gene
Purification
≥80%
Tested applications
SDS PAGE, N/A
Level of endotoxin
None
Tested activity
≥ 1.0 x 106 KU/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit (U) of Binuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min under the condition above, which means complete digestion of 37 μg DNA
Storage condition
4°C
Shipping under
Gel Pack
Physical appearance
Liquid
Physical properties
Liquid: clear colorless buffered aqueous glycerol solution
How to reconstitute
20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 2 mM NaCl, 50% Glycerol
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans