Isocitrate Dehydrogenase 1 R132H mutant, Recombinant Human
Catalog number
P1070-100
Name
Isocitrate Dehydrogenase 1 R132H mutant, Recombinant Human
Size
100 μg
Price
512.00 EUR
Supplier
Details
Background
Mutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.
Concentration
N/A
Molecular weight
50.9 kDa
Synonym
Isocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
Other name
Isocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
NCBI gene number
3417
NCBI gene
IDH1
Gene source
Human
NCBI number
O75874
Recombinant
Yes
Source
E. Coli
Purification
≥90%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
≥ 300 mU/mg, in which one unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
Reesults
≥ 300 mU/mg
Binding ability
N/A
Unit
One unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
N/A
How to reconstitute
Reconstitute in deionized water to the final concentration 0.5 mg/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
