Human CellExp™ B7-1 / CD80, mouse recombinant

CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA- 4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

N/A

25.6 kDa

CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

12519

Cd80

Mouse

Q00609

Yes

HEK 293 cells

>95%

SDS-PAGE, N/A

< 1 EU/μg

Measured by its binding ability in a functional ELISA

Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.

N/A

Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.

N/A

-20°C

Gel pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

Recombinants or rec. proteins

recombinants