Hygromycin B for cell activation

Catalog number
2589-500
Name
Hygromycin B for cell activation
Size
500 mg
Price
197.00 EUR
Supplier

Details

Description
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus that is routinely used as a selection agent for cells that contain hph or hyg Hygromycin resistant gene. Hygromycin B is thought to inhibit protein synthesis by strengthening the interaction of peptidyl-tRNA in the ribosomal acceptor (“A”) site thereby preventing translocation of by EF-2 (elongation Factor-2). Hygromycin B is effective on most bacteria, fungi and higher eukaryotes. 
Peptide sequence
N/A
CAS number
31282-04-9
Molecular weight
527.52
Category
Biochemicals
Other name
 (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Molecular formula
C₂₀H₃₇N₃O₃
Physical appearance
Off-white to light tan powder
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥90% by HPLC
Reconstitute instructions
H₂O (~ 50 mg/ml)
Storage condition
2-8ºC
Shipping condition
Gel pack
Maximum time for storage
36 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.