IWP-4 for cell activation

IWP-4 is a potent inhibitor of Wnt production that blocks Wnt pathway activity in vitro (IC₅₀ = 25 nM). IWP-4 inactivates Porcupine (porcn), a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. At 5 μM, IWP-4 has been shown to block Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 receptor and the scaffold protein Dishevelled, preventing the accumulation of β-catenin. IWP-4 has been used to induce cardiomyocyte differentiation from human pluripotent stem cells.

N/A

686772-17-8

496.62

Biochemicals

N-​(6-​methyl-​2-​benzothiazolyl)-​2-​[[3,​4,​6,​7-​tetrahydro-​3-​(2-​methoxyphenyl)-​4-​oxothieno[3,​2-​d]pyrimidin-​2-​yl]thio]-​acetamide

C₂₃H₂₀N₄O₃S₃

Crystaline solid

N/A

No

Yes

≥95% by HPLC

DMSO (~2 mg/ml)

-20°C

gel pack

36 months

Protect from air and light

cell

For cells, cell lines and tissues in culture till half confluency.