EZSolution™ DAF-2 for cell activation

DAF-2 is a highly sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.

N/A

205391-01-1

362.34

Biochemicals

4,5-Diaminofluorescein; 2-​(3,​6-​dihydroxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid

C₂₀H₁₄N₂O₅

Liquid

In DMSO (5 mM)

No

No

≥95% by HPLC

N/A

-20°C

Gel Pack

36 months

Protect from air and light

cell

For cells, cell lines and tissues in culture till half confluency.