EZSolution™ DAF-2 diacetate for cell activation

DAF-2 diacetate is a cell-permeable derivative of DAF-2 (Cat. No. 2752) and a very useful indicator of nitric oxide (NO). Upon entry into the cell, DAF-2 diacetate is converted into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.

N/A

205391-02-2

446.41

Biochemicals

4,5-Diaminofluorescein diacetate; 2-​(3,​6-​diacetyloxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid; DAF-2 DA

C₂₄H₁₈N₂O₇

Liquid

In DMSO (5 mM)

No

Yes

≥98% by HPLC

N/A

-20°C

Gel Pack

36 months

Protect from air and light

cell

For cells, cell lines and tissues in culture till half confluency.