FITC-VAD-FMK for cell activation

FITC-VAD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable pan-caspase inhibitor VAD(OMe)-FMK that acts as an in situ marker for detection of caspase in living cells. FITC-VAD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase in apoptotic cells. The bound marker is localized by fluorescence detection.

FITC-VAD(OMe)-FMK

N/A

722.74

Biochemicals

FITC-Val-Ala-Asp- fluoromethyl ketone; FITC-VAD(OMe)-FMK

C₃₅H₃₅FN₄O₁₀S

Liquid

A 0.6 mM solution in DMSO

No

Yes

≥98% by HPLC

N/A

-20°C

gel pack

36 months

Protect from air and moisture

cell

For cells, cell lines and tissues in culture till half confluency.

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody