FITC-VAD-FMK for cell activation

Catalog number
9497-100
Name
FITC-VAD-FMK for cell activation
Size
100 assays
Price
360.00 EUR
Supplier

Details

Description
FITC-VAD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable pan-caspase inhibitor VAD(OMe)-FMK that acts as an in situ marker for detection of caspase in living cells. FITC-VAD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase in apoptotic cells. The bound marker is localized by fluorescence detection.
Peptide sequence
FITC-VAD(OMe)-FMK
CAS number
N/A
Molecular weight
722.74
Category
Biochemicals
Other name
FITC-Val-Ala-Asp- fluoromethyl ketone; FITC-VAD(OMe)-FMK
Molecular formula
C₃₅H₃₅FN₄O₁₀S
Physical appearance
Liquid
Supplied with
A 0.6 mM solution in DMSO
Is this a salt?
No
Is it cell-permeable?
Yes
Purification
≥98% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
36 months
Storage instructions
Protect from air and moisture
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.
Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody