Skepinone-L for cell activation

Skepinone-L is a potent, ATP-competitive inhibitor of p38 MAPK isoform p38α (IC₅₀ = 5 nM) and p38β (97% inhibition at 1 µM). It has little effect on a range of other kinases, including p38γ and p38δ. Skepinone-L dose-dependently blocks the phosphorylation of heat shock protein 27 (HSP27), a p38 MAPK substrate, in response to stimulation with anisomycin (Cat. No. 1549) in HeLa cells (IC₅₀ = 25 nM) or TNF-α in THP-1 cells.

N/A

1221485-83-1

425.4

Biochemicals

2-[(2,4-difluorophenyl)amino]-7-[(2R)-2,3-dihydroxypropoxy]-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5-one

C₂₄H₂₁F₂NO₄

Yellow solid

N/A

No

Yes

≥98% by HPLC

DMSO (~15 mg/ml)

-20°C

gel pack

36 months

Protect from air and light

cell

For cells, cell lines and tissues in culture till half confluency.