Azidothymidine for cell activation

Azidothymidine is a nucleoside reverse transcriptase inhibitor that, following triphosphorylation by thymidine kinase, potently blocks replication of HIV (EC₅₀ = 3 nM) with low cytotoxicity (CC₅₀ > 5 μM). Displays 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. Suppresses HIV-1 replication and enhances cell viability in HIV-1 infected T cells. Antiretroviral agent. Also decreases CRISPR-mediated homology directed repair (HDR) efficiency.

N/A

30516-87-1

267.24

Biochemicals

3'-Azido-3'-deoxythymidine; AZT; Zidovudine

C₁₀H₁₃N₅O₄

White solid

N/A

No

Yes

≥98% by HPLC

DMSO (>25 mg/ml) or H2O (>10 mg/ml)

-20°C

gel pack

36 months

Protect from air and moisture

cell

For cells, cell lines and tissues in culture till half confluency.