EZBlock™ Protease Inhibitor Cocktail VIII, DMSO-Free for cell activation

A cocktail of five protease inhibitors with broad specificity for the inhibition of aspartic, serine, cysteine, aminopeptidases and thermolysin-like proteases. This cocktail is recommended for purification of proteins containing His-Tag sequences. One ml of the reconstituted cocktail is recommended for about 10 g of cells. Each vial contains 100 mM of AEBSF, HCl (Cat. No.1644), 5 mM of Bestatin (Cat. No. 1733), 1.5 mM of E-64 (Cat. No 1739), 2 mM of Pepstatin A (Cat. No. 1732) and 200 µM of Phosphoramidon disodium salt.

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Biochemicals

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Lyophilized solid

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-20°C

gel pack

24 months

Protect from air and moisture

cell

For cells, cell lines and tissues in culture till half confluency.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

This inhibitor of proteases will block aspartic protease, cysteine protease, serine proteases, metalloproteases and aminopeptidases. Lyophilized, EDTA free.