DiscoveryPak™ Stem Cell Fate Regulator Set V for cell activation

A convenient set of five small molecules useful for generating human induced pluripotent stem cells (iPSC) from primary cells. The five products are: 1 mg of A-83-01 (Cat. No. 1725-1), 2 mg of PD-0325901 (Cat. No. 1643-2), 5 mg of PS48 (Cat. No. 1869-5), 1 g of Sodium Butyrate (Cat. No. 1609-1000) and 25 mg of Tranylcypromine hemisulfate (Parnate) (Cat. No. 1816-25).

N/A

See under the individual product

See under the individual product

Biochemicals

Stem Cell Fate Regulator Set V

See under the individual product

Lyophilized solids

N/A

No

Yes

≥95% by HPLC

See under the individual product

-20°C

gel pack

24 months

Protect from air, light and moisture

cell, set, stem

For cells, cell lines and tissues in culture till half confluency.

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.