DiscoveryPak™ Stem Cell Fate Regulator Set VIII (VC6T) for cell activation

Catalog number
K887-4
Name
DiscoveryPak™ Stem Cell Fate Regulator Set VIII (VC6T) for cell activation
Size
4 modulators
Price
447.00 EUR
Supplier

Details

Description
A convenient set of four small molecule modulators (V6CT) useful for generation of iPSC’s (induced pluripotent stem cells) from mouse fibroblasts using a single gene, Oct4. The four modulators are: 200 mg of Valproic acid sodium salt (VPA) (Cat. No. 1647-200), 5 mg of CHIR99021 (Cat. No. 1677-5), 5 mg of RepSox (E-616452) (Cat. No. 1894-5) and 25 mg of Tranylcypromine Hemisulfate (Parnate) (Cat. No.1816-25).
Peptide sequence
N/A
CAS number
See under the individual item
Molecular weight
See under individual item
Category
Biochemicals
Other name
VC6T
Molecular formula
See under individual item
Physical appearance
Lyophilized solids
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
Y
Purification
See under individual item
Reconstitute instructions
See under the individual product
Storage condition
-20°C
Shipping condition
Gel Pack
Maximum time for storage
36 months
Storage instructions
Protect from air and light
Tissue
cell, set, stem
Additional description
For cells, cell lines and tissues in culture till half confluency.
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.