Thioredoxin 1 (Trx) Fluorometric Substrate, Di- FITC oxidized glutathione (FGSSGF) for cell activation

Ready-to-use fluorogenic substrate for Trx. Trx activity can be quantified by fluorescent detection of oxidized Glutathione after reduction of the disulfide bond at 490/525 nm, using a fluorometer or multi-well fluorescence plate reader.

FITC-Glutathione-Glutathione-FITC

N/A

1391.36

Trx substrate

N/A

Lyophilized

N/A

No

>99% by HPLC

Resuspend 835 µg in 6 ml of 50 mM sodium phosphate buffer at pH 7.4.

+4°C

gel pack

12 months

Store at 4°C, protected from light.

cell

For cells, cell lines and tissues in culture till half confluency.

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody