Annexin V-FITC Apoptosis Kit

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.

• Detection method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

• Simple one step staining procedure in 10 minutes • Fast and convenient • Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining

Apoptosis Assay Kit

none

• Annexin V-FITC • 1X Binding Buffer • Propidium Iodide (PI)

+4°C

gel pack

12 months

Mammalian

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Living cells (suspension and adherent)

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody