Annexin V-Cy3 Apoptosis Kit

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

• Detection method: Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays)

• Simple one step staining procedure in 10 minutes • Fast and convenient • Cy3 shows brighter red fluorescence

Apoptosis Assay Kit

none

• Annexin V-Cy3 • 1X Binding Buffer

+4°C

gel pack

12 months

Mammalian

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Living cells (suspension and adherent)

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Annexin V- Apoptosis Kit can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Biovision antibody is well suited for fluorescent microscopy.

cy3 conjugation kit