Annexin V-Cy5 Apoptosis Kit

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

• Detection method: Flow cytometry (Ex = 649 nm; Em = 670 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays)

• Simple one step staining procedure in 10 minutes • Fast and convenient • Annexin V-Cy5 will show bright red-blue staining on the plasma membrane

Apoptosis Assay Kit

none

• Annexin V-Cy5 • 1X Binding Buffer

+4°C

gel pack

12 months

Mammalian

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Living cells (suspension and adherent)

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.

Cy5.5