Annexin V-Cy5 Apoptosis Kit

Catalog number
K103-25
Name
Annexin V-Cy5 Apoptosis Kit
Size
25 assays
Price
227.00 EUR
Supplier

Details

Kit's description
Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
Highlights
• Detection method: Flow cytometry (Ex = 649 nm; Em = 670 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays)
Kit's benefits
• Simple one step staining procedure in 10 minutes • Fast and convenient • Annexin V-Cy5 will show bright red-blue staining on the plasma membrane
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Annexin V-Cy5 • 1X Binding Buffer
Storage condition
+4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Living cells (suspension and adherent)
Description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.
Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Conjugation
Cy5.5