Annexin V-EGFP Apoptosis Kit

The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

• Detection method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: 25, 100, 400 • Applications:Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

• Simple one step staining procedure in 10 minutes • Fast and convenient • EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining

Apoptosis Assay Kit

none

• Annexin V-EGFP • 1X Binding Buffer • Propidium Iodide (PI)

+4°C

gel pack

12 months

Mammalian

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Living cells (suspension and adherent)

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

The Green Fluorescent Protein (eGFP) of Annexin V- Apoptosis Kit can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.