Caspase-8 Colorimetric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

• Detection method: Absorbance (400 or 405 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.

Apoptosis Assay Kit

none

• Cell Lysis Buffer • 2X Reaction Buffer • IETD-pNA (4 mM) • DTT (1 M) • Dilution Buffer

-20°C

gel pack

12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Cell and tissue lysates

Biovision supplies other types of Assays as 1.

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.