Annexin V-PE-Cy5 Apoptosis Detection Kit

The Annexin V-PE-Cy5 Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.

• Detection method: Flow cytometry (Ex = 488 nm; Em = 670 nm) and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

• Simple one-step procedure; takes only 10 minutes • Fast and convenient, no need of fixation of cells

Apoptosis Assay Kit

none

• Annexin V-PE-Cy5 • 1X Binding Buffer

+4°C

gel pack

12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Live cells

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.

Cy5.5