Caspase Fluorometric Substrate Set Plus
Catalog number
K135-7-25
Name
Caspase Fluorometric Substrate Set Plus
Size
7 x 25 assays
Price
702.00 EUR
Supplier
Details
Kit's description
Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
Highlights
• Detection method: Fluorescence (Ex/Em 400/505) • Species reactivity: Mammalian • Kit size: 7x25 assays • Applications: Detects specific Caspses involved in apoptosis within cells
Kit's benefits
• Contains ready-to-use caspase-1/-2/-3/-5/-6/-8/-9 AFC-labeled substrates and all buffers for performing caspase assay
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Caspase-1 Substrate, Ac-YVAD-AFC • Caspase-2 Substrate, Ac-VDVAD-AFC • Caspase-3 Substrate, Ac-DEVD-AFC • Caspase-5 Substrate, Ac-WEHD-AFC • Caspase-6 Substrate, Ac-VEID-AFC • Caspase-8 Substrate, Ac-IETD-AFC • Caspase-9 Substrate, Ac-LEHD-AFC • Cell Lysis Buffer • 2X Reaction Buffer • DTT (1 M)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
6-12 months
Species reactivity
see datasheet
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Cell culture
Tissue
set
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
