Caspase Colorimetric Substrate Set Plus

Catalog number
K136-7-25
Name
Caspase Colorimetric Substrate Set Plus
Size
7 x 25 assays
Price
702.00 EUR
Supplier

Details

Kit's description
Ready-to-use colorimetric substrates for members of caspase family proteases. All substrates were provided in liquid ready-to-use form.
Highlights
• Detection method: Absorbance (400 or 405 nm ) • Species reactivity: Mammalian • Kit size: 7X25 assays • Applications: Detects specific Caspases involved in apoptosis within cells
Kit's benefits
• Contains ready-to-use caspase-1/-2/-3/-5/-6/-8/-9 pNA-labeled substrates and all buffers for performing caspase assay
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Caspase-1 Substrate, Ac-YVAD-pNA • Caspase-2 Substrate, Ac-VDVAD-pNA • Caspase-3 Substrate, Ac-DEVD-pNA • Caspase-5 Substrate, Ac-WEHD-pNA • Caspase-6 Substrate, Ac-VEID-pNA • Caspase-8 Substrate, Ac-IETD-pNA • Caspase-9 Substrate, Ac-LEHD-pNA • Cell Lysis Buffer • Dilution Buffer • 2X Reaction Buffer • DTT (1 M)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
6-12 months
Species reactivity
see datasheet
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Cell culture
Tissue
set
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.