Caspase Colorimetric Substrate Set II Plus

Ready-to-use colorimetric substrates for members of caspase family proteases. All substrates were provided in liquid ready-to-use form.

• Detection method: Absorbance (400 or 405 nm ) • Species reactivity: Mammalian • Kit size: 9X25 assays • Applications: Detects specific Caspases involved in apoptosis within cells

• Contains ready-to-use caspase-1/-2/-3/-4/-5/-6/-8/-9/-10 pNA-labeled substrates and all buffers for performing caspase assay

Apoptosis Assay Kit

none

• Caspase-1 Substrate, Ac-YVAD-pNA • Caspase-2 Substrate, Ac-VDVAD-pNA • Caspase-3 Substrate, Ac-DEVD-pNA • Caspase-4 Substrate, Ac-LEVD-pNA • Caspase-5 Substrate, Ac-WEHD-pNA • Caspase-6 Substrate, Ac-VEID-pNA • Caspase-8 Substrate, Ac-IETD-pNA • Caspase-9 Substrate, Ac-LEHD-pNA • Caspase-10 Substrate, Ac-AEVD-pNA • Cell Lysis Buffer • Dilution Buffer • 2X Reaction Buffer • DTT (1 M)

-20°C

gel pack

6-12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Cell culture

set

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.