Cathepsin B Activity Fluorometric Assay Kit

Catalog number
K140-100
Name
Cathepsin B Activity Fluorometric Assay Kit
Size
100 assays
Price
480.00 EUR
Supplier

Details

Kit's description
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
Highlights
• Detection method: Fluorescence (Ex/Em 400/505) and fluorometer • Species reactivity: Mammalian • Kit size: 100 assays • Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
Kit's benefits
• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Cathepsin-B assay is straightforward, and can be adapted to 96-well plate assays.
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• CB Cell Lysis Buffer • CB Reaction Buffer • CB Substrate Ac-RR-AFC (10 mM) • CB Inhibitor (1 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
6-12 months
Species reactivity
see datasheet
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Cell and tissue lysates
Test
Biovision supplies other types of Assays as 1.
Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.