Cathepsin K Activity Fluorometric Assay Kit

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-K Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

• Detection method: Fluorescence (Ex/Em 400/505) • Species reactivity: Mammalian • Kit size: 100 assays • Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Cathepsin-K assay is straightforward, and can be adapted to 96-well plate assays.

Apoptosis Assay Kit

none

• CK Cell Lysis Buffer • CK Reaction Buffer • CK Substrate Ac-LR-AFC (10 mM) • CK Inhibitor (1 mM)

-20°C

gel pack

6-12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Cell and tissue lysates

Biovision supplies other types of Assays as 1.

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.