Cathepsin G Activity Fluorometric Assay Kit

Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In BioVision’s Cathepasin G Activity assay Kit, Cathepsin G will cleave the substrate and release the dye group, pNA (4-Nitroaniline), which can be detect at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable for the activity of Cathepsin G.

• Detection method: Absorbance (405 nm) • Species reactivity: Mammalian • Kit size: 100 assays • Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.

• Simple one-step procedure; takes only 1 hours • Fast, robust and convenient • Cathepsin-G assay is straightforward, and can be adapted to 96-well plate assays.

Apoptosis Assay Kit

none

• Assay Buffer • Substrate • Cathepsin G Inhibitor • pNA Standard (0.1 M) • Positive Control

-20°C

gel pack

6-12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Cell and tissue lysates, serum

Biovision supplies other types of Assays as 1.

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.