Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric)

Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis. Biovision’s Cathepsin B Inhibitor Screening Kit utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin B.

• Detection method: Fluorescence (Ex/Em = 400/505 nm) • Applications: Screen/study/characterize potential inhibitors of Cathepsin B.

• Simple & convenient • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin B • Cathepsin B inhibitor, F-F-FMK is included in the kit

Cathepsin B

none

• CTSB Reaction Buffer • CTSB Reagent • Cathepsin B (human) • CTSB Substrate, Ac-RR-AFC (10 mM) • CTSB Inhibitor (F-F-FMK, 1 mM)

-20°C

gel pack

12 months

see datasheet

Screen/study/characterize potential inhibitors of Cathepsin B.

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.